Your reconstitution copilot
Vial contents
Serum concentration
Final serum batch
Reconstitution
Dose
Draw to
on a 1.0 mL insulin syringe
Volume
0.05 mL
Concentration
5.00 mg/mL
Total doses
20
Lasts
20 days
Add to your serum base
of peptide for a 30 mL serum at 0.5%
- Reconstitute the calculated peptide amount in a small volume of bacteriostatic water (or your hydrating ingredient).
- Add to your prepared serum base and mix gently — don't shake.
- Adjust pH to 5–6 if formulating with hyaluronic acid; check with strips.
- Transfer to a sterile dropper or pump bottle. Refrigerate when not in use.
- Use within 4–6 weeks. Discard if color, smell, or texture changes.
Reconstitution procedure
- Bring vial to room temperature Remove the lyophilized peptide from cold storage 15–30 minutes before reconstitution. Reduces condensation when the stopper is pierced.
- Sanitize stoppers Wipe the rubber stoppers on both the peptide vial and the bacteriostatic water vial with a fresh alcohol prep pad. Allow to air-dry — don't blow on it.
- Draw the calculated BAC volume Use a sterile syringe (a 3 mL syringe with a longer needle is convenient for this step). Pull the calculated milliliters of bacteriostatic water — see the result above.
- Inject down the inside wall, slowly Insert the needle into the peptide vial and angle the stream against the inner glass wall — not directly onto the powder. Slow injection minimizes foaming and protein denaturation.
- Swirl, never shake Roll the vial gently between your palms or move in slow circles until the powder fully dissolves. Vigorous shaking creates shear stress that can damage peptide bonds.
- Inspect the solution The reconstituted solution should be clear and colorless. Visible particulates, cloudiness, or discoloration are signs to discard the vial.
- Date and label Write the reconstitution date on the vial. Most peptides remain stable for the shelf-life window set above when refrigerated. Verify the specific peptide's stability data — some are shorter (e.g., reconstituted Tesamorelin per its label).
- Store and use Refrigerate at 2–8°C (35–46°F), away from light. For each injection, allow the vial to reach room temperature, sanitize the stopper, draw the calculated units, rotate injection sites.
Show the math
Terms & Disclaimers
1. Research and educational use only
PeptPilot is a calculator for reconstitution arithmetic and an educational reference about peptide compounds. Nothing in this app constitutes medical advice, diagnosis, or treatment. Use of any peptide on yourself, another person, or any living subject is your own responsibility.
2. Not a substitute for professional medical care
Dosing, frequency, and protocol information shown is drawn from publicly available pharmacology literature, FDA prescribing documents (where applicable), and ranges commonly cited in research communities. Many peptides referenced are not FDA-approved for human use in the United States. Always consult a qualified, licensed medical provider before any peptide use, especially if you take other medications, are pregnant or nursing, or have underlying health conditions.
3. Confidence ratings explained
- A — FDA-approved for human use; well-characterized pharmacokinetics
- B — Investigational or in clinical trial stages with peer-reviewed human data
- C — Research peptide with primarily preclinical or limited human data
- D — Speculative; narrow source base or limited independent replication
4. Math accuracy
The calculator uses standard reconstitution math (units = dose ÷ vial × BAC × 100). All inputs and outputs should be independently verified before any decision is made based on them. Floating-point arithmetic introduces tiny rounding differences which are normally insignificant but should not be relied on for critical applications.
5. No data collection
PeptPilot runs entirely in your browser. No values you enter, peptides you select, or settings you configure are transmitted to a server. The donate button opens Square's hosted checkout in a new tab; Square handles that transaction independently.
6. No liability
Use of this app is at your own risk. The authors and operators of PeptPilot accept no liability for any harm, injury, illness, or other adverse outcome arising directly or indirectly from use of the calculator, the reference data, or any decisions made based on either. By using PeptPilot you agree to hold the authors and operators harmless from any such claims.
7. Compliance with local law
Possession, sale, and use of peptide compounds is regulated differently in different jurisdictions. You are responsible for understanding and complying with the laws applicable to you. PeptPilot is provided globally for educational purposes only and does not endorse, encourage, or facilitate any unlawful activity.
8. Changes to these terms
These terms may be updated at any time. Continued use of PeptPilot constitutes acceptance of the current version.
9. Contact
For questions about these terms, use the donation link footer to reach the operator through your purchase confirmation, or report issues through the feedback channels provided.