Beta

Your reconstitution copilot

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⚠
Research and educational use only. PeptPilot performs reconstitution arithmetic — it is not medical advice. Many peptides shown are not FDA-approved. Read the full terms.

Vial contents

Physical vial size

Topical peptide

Cosmetic / topical ingredients only
Ingredient form
Topical mode assumes cosmetic/topical-grade powder or a supplier-designated topical ingredient. Do not assume a sterile injectable or research vial is appropriate for skin-care formulation.

Serum concentration

Concentration (% w/v)
%
Common concentrations
For branded cosmetic solutions, use the supplier usage rate instead of raw powder percent.

Final serum batch

Final volume
mL
Common bottle sizes

Serum base

Beginner pick Use a premade, unscented, water-based serum or gel base from a cosmetic supplier.
What to look for A preserved base with supplier guidance for pH, compatibility, and how much active ingredient can be added.
Avoid Oil-only bases unless the supplier says compatible, BAC water as a skincare preservative, or unpreserved water batches.

Reconstitution

Set by
BAC water
mL
Suggested BAC volumes (cleanest math first)

Dose

Per injection
Peptide 2 dose
Syringe size
Frequency
Reconstituted shelf life
days

Draw to

25 units

on a 1.0 mL insulin syringe

⚠ Compatibility caution

Volume

0.05 mL

Concentration

5.00 mg/mL

Total doses

20

Lasts

20 days

Storage timer

For the reconstituted peptide vial. BAC first-puncture date is only used to calculate the sterility reminder.

Optional

Saved on this browser. Sign in to sync across devices, or use share for a copy.

Reconstituted date
BAC first puncture
Override window
days

Add dates to show storage guidance.

PeptPilot defaults to a 28-day BAC water sterility window after first puncture.

Saved reconstituted vials on this device

No saved reconstituted vials yet. Add dates, then save the vial record.

Add to your serum base

150 mg

of peptide for a 30 mL serum at 0.5%

150 mg
Powder
30 mL
Serum batch
Final concentration 5.0 mg/mL
Containers needed 3 x 50 mg
Carrier base volume ~30 mL
Batch yield 30 mL
  1. Use only cosmetic/topical-grade peptide powder or a supplier-designated topical ingredient.
  2. Add to your prepared serum base and mix gently — don't shake.
  3. Adjust pH to 5–6 if formulating with hyaluronic acid; check with strips.
  4. Transfer to a sterile dropper or pump bottle. Refrigerate when not in use.
  5. Use within 4–6 weeks. Discard if color, smell, or texture changes.
Reconstitution procedure
  1. Bring vial to room temperature Remove the lyophilized peptide from cold storage 15–30 minutes before reconstitution. Reduces condensation when the stopper is pierced.
  2. Sanitize stoppers Wipe the rubber stoppers on both the peptide vial and the bacteriostatic water vial with a fresh alcohol prep pad. Allow to air-dry — don't blow on it.
  3. Draw the calculated BAC volume Use a sterile syringe (a 3 mL syringe with a longer needle is convenient for this step). Pull the calculated milliliters of bacteriostatic water — see the result above.
  4. Inject down the inside wall, slowly Insert the needle into the peptide vial and angle the stream against the inner glass wall — not directly onto the powder. Slow injection minimizes foaming and protein denaturation.
  5. Swirl, never shake Roll the vial gently between your palms or move in slow circles until the powder fully dissolves. Vigorous shaking creates shear stress that can damage peptide bonds.
  6. Inspect the solution Most reconstituted peptide solutions should be clear and colorless. GHK-Cu and blends containing GHK-Cu are expected to look blue because of the copper complex. Visible particulates, cloudiness, unexpected color changes, or unusual residue are signs to discard or review the vial.
  7. Date and label Write the reconstitution date on the vial. Most peptides remain stable for the shelf-life window set above when refrigerated. Verify the specific peptide's stability data — some are shorter (e.g., reconstituted Tesamorelin per its label).
  8. Store and use Refrigerate at 2–8°C (35–46°F), away from light. For each injection, allow the vial to reach room temperature, sanitize the stopper, draw the calculated units, rotate injection sites.
Storage guide

The short version

PeptPilot separates two different ideas: peptide chemical stability and multi-dose sterility risk. A peptide molecule does not automatically disappear on day 29, but a reconstituted vial can become a sterility problem before the peptide itself is chemically gone.

The app uses the conservative rule: once BAC water is punctured, the default reminder window is 28 days. That date is treated as a sterility reminder, not proof of peptide degradation.

Powder vs liquid

Lyophilized peptide powder is the durable form. Keep it sealed, dry, cold, and protected from light. Let a cold vial warm in its packaging before handling so condensation does not form on the stopper or powder cake.

Reconstituted peptide is different. Once water is introduced, hydrolysis, oxidation, handling error, and microbial risk become active concerns. Refrigeration slows problems down, but it does not make a multi-dose vial permanently sterile.

BAC water timer

Bacteriostatic water contains benzyl alcohol to inhibit bacterial growth in a multi-dose vial. It is not a guarantee that contamination cannot happen, and it is not a peptide preservative in the way many people assume.

  • Record the first puncture date on the BAC water vial.
  • Use fresh sterile needles every time.
  • Alcohol-swab stoppers before each puncture and let them dry.
  • PeptPilot defaults to 28 days after first BAC puncture for the sterility reminder.

The stricter date wins

A reconstituted vial can have more than one clock running: the reconstitution date, the BAC first-puncture date, and peptide-specific handling concerns. PeptPilot recommends the earliest relevant date by default.

Example: if BAC water was first punctured 20 days ago and used to mix a vial today, the app treats the BAC window as having roughly 8 days left unless the user overrides the reminder.

Override policy

The override exists because adults may choose to track what they actually do. Turning it on only extends the reminder date. It does not mean PeptPilot believes the vial is sterile, safe, or appropriate to inject.

When override is active, PeptPilot keeps the warning visible: sterility, preservative effectiveness, and contamination risk cannot be verified by this app.

When to discard

  • Cloudiness, particles, unexpected color change, or unusual residue appear. GHK-Cu is the common exception where a blue tint is expected.
  • The vial was left at an uncertain temperature for an uncertain amount of time.
  • The stopper was touched, punctured with questionable technique, or reused with a non-sterile needle.
  • The vial is past the default sterility window and you are not intentionally overriding that risk.
Show the math
Awaiting valid inputs.

Guides

Using PeptPilot

Start with the calculator. Inject is for one reconstituted vial, Blend is for two peptides in the same vial, and Topical is for cosmetic serum batch math.

Enter the vial or ingredient amount, choose the mode that matches what you are doing, then review the result before using it.

Storage and BAC timer

The storage timer tracks the BAC water sterility reminder separately from peptide shelf life. The default 28-day timer is conservative, not proof that a vial is sterile or unsafe.

  • Save each mixed vial separately.
  • Use the first BAC puncture date when you know it.
  • Override only changes the reminder date; it does not verify sterility.
Topical beginner path

For beginners, the simplest topical path is a premade, preserved, water-based serum or gel base from a cosmetic supplier.

  • Use Powder mode when you have cosmetic/topical-grade powder.
  • Use Supplier Solution mode when the ingredient is already diluted and has a usage-rate label.
  • Avoid using BAC water as a skincare preservative plan.
GHK-Cu color and copper balance

GHK-Cu is a copper-bound peptide, so a blue solution can be normal. Cloudiness, particles, green/brown shifts, or unexpected fading are different from the expected blue tint and should be treated as a reason to review or discard.

Some communities describe temporary skin worsening as "copper uglies." Published evidence is limited, so PeptPilot treats it as an anecdotal caution rather than a proven diagnosis. People also discuss zinc because copper and zinc balance each other biologically, but aggressive supplementation can create its own problems. Use labs and clinician guidance when copper or zinc status matters.